OC-023 USING FIBROBLAST MEDIATED MATRIX CONTRACTION TO TEST MESH SHRINKAGE - A PROTOTYPE FOR IN VITRO MESH TESTING

Abstract Background/Aim Measuring how hernia meshes shrink after implantation is a commonly used parameter in vivo mesh testing. We aim to use fibroblast populated collagen matrices (FPCMs) and their contraction assess different shrink. Methods/Materials 1 millilitre circular FPCMs were formed 24 well plates. Different placed halfway through the so that they embedded. Matrices seeded with immortalised human fibroblasts, allowed set then separated form plate using sterile needle. Gels cultured media at 37 degrees 5% CO2 for seven days contract naturally. Each day, photographs taken area of calculated. Results When comparing with/without mesh, It was noted significantly greater observed without - which hampered contraction. meshes, lightweight larger pores lead (54% Vs 45%) shrinkage (55% 20%) compared heavyweight smaller pores. Conclusion present cheap, standardised unique technique measuring vitro.